EFTA00367486.pdf

From: Joseph Thakuria To: Lesley Groff Subject: Re: Jeffrey Epstein 1:30 Sat. June 21st? Date: Mon, 23 Jun 2014 17:48:47 +0000 Thanks - most ypo folks also enrolled a spouse and/or other family. Some also offered it to their employees. Joe On Jun 23, 2014 11:41 AM, "Lesley Groff' < > wrote: thank you! Will pass along to JE and try to see what he intends to do with the extra 4 kits asap! On Jun 23, 2014, at 1:28 PM, Joseph Thakuria < > wrote: Lesley, thanks for the email. Jeffrey, can add whoever he want into the YPO exome study but I will need to have those samples mailed to me directly and received by next Monday at the latest to be included in this sequencing run. The exomes (at cost) are $1000 each. I'll add this to his invoice which I'll send before the end of the week. I don't want this getting lost or held up in HMS or MGH interoffice mail. So, please mail to my home address: Joe Thakuria He should have (if I remember correctly) 4 extra kits. If he needs more, let me know right away because we'll be cutting it close in terms of getting into this round (with mailing it out to him and then back to me). The instructions in the pack are straightforward and he just did it recently but, to summarize: I. do not eat/drink/smoke or chew gum for 30minutes prior to collecting (eg, if you ate a chili dog, we could get contamination from pork, beef, chicken, tomato, kidney bean, and chili pepper genomes; you'd be surprised how much similarity there is on the DNA level between very different species) 2. spit into the funnel and fill to the indicated line (not all the way to the top so there's room for #3) and make sure this isn't filled with bubbles. If there are bubbles it may need to settle a little so the process typically takes 2 - 5 minutes. 3. There are 2 caps. When done spitting, close the first cap (attached to the funnel the person spits into) so that you hear a click and the liquid in that first cap goes into the tube. That liquid needs to mix with the collected saliva. 4. Then take that first cap off an put the second smaller cap on and close tightly. Make sure the cap is on well and not leaking (or the sample can't be used). The following are VERY IMPORTANT: 5. You or someone else will need to send me the names and contact info (especially email and phone) for people who are submitting samples. EFTA00367486 6. But do not put those names on the tubes or fill out paperwork that came with the tubes. I'll take care of that. 7. You do need to label the tubes though. For the additional 4 samples, you can use the following designations: YPO 76, YPO 77, YPO 78, YPO 79 8. Obviously I will need to know who is who. (So you'll need to email me: "YPO 76 is jane doe, YPO 77 is john doe, etc.) I can't overemphasize the importance of the labeling. If I'm given the YPO number, names, and/or samples are incorrectly matched, the people will get results that are not theirs. It's actually very straightforward but I want to avoid any mistakes by spelling everything out. You can mail the tubes back in the boxes (and unfilled paperwork) they came in. But, of course, make sure each tube is labeled with the appropriate YPO # so we know who is who. Email me with any questions. Thanks! Joe On Mon, Jun 23, 2014 at 11:29 AM, Lesley Groff <1 > wrote: HI Joe...I see you wanted to provide Jeffrey with some instruction on the kits today...Let me know! thanks, Lesley On Jun 21, 2014, at 3:58 PM, Joseph Thakuria > wrote: Hi LesleyM, and (and Jeffrey), a, as you know I left 4 saliva collection kits as Jeffrey requested. I'm ordering more, so let me know if he needs more. The study will be open indefinitely but sample collection for this round of sequencing (which includes -80 YPO Austin folks) will close in 1-2 weeks. (So probably a few months wait if this window is missed.) If it's not too much trouble, hold off on collecting additional samples with those 4 kits until Monday. On Monday, I'll provide instructions on mailing and labeling those kits if Jeffrey wants to use those for include more people in this pilot YPO phase of the study. Jeffrey, it was good seeing you again today and chatting, however briefly, and I look forward to discussing this project with you more at your convenience. Let me put together a very basic invoice for the sequencing we'll be doing for you (beyond what the other ypo people are having done, including the $11,000+ whole genome from Illumina). I'll also include some basic infrastructure to pursue that specific genomic project you mentioned briefly. I'd be happy to help with that but we should discuss first (after I put together an invoice for hardware/software and maybe 1 bioinformatics hire) the best way to make this happen. EFTA00367487 Safe travels back to NY, Joe Thakuria On Wed, Jun 18, 2014 at 11:15 AM, Lesley Groff MIEla wrote: tremendous...thanks On Jun 18, 2014, at 11:15 AM, Joseph Thakuria < Thanks, Lesley - confirmed for Sat. 1:30. ME/=, I'll call when I get there. Joe wrote: Hello Joe...I meant to cc and on my previous mail to you...these are the assistants who will accompany Jeffrey to Harvard this Sat. Please reply to all of us re if you can make 1:30pm on Sat. June 21st at Martin Nowak's Institute (I Brattle Square, Suite 6) to take Jeffrey's saliva sample... Assuming you can make it!- please call or upon your arrival to the institute so they can come down and let you in... I am leaving on holiday today and will have limited cell service/email service... thanks, Lesley EFTA00367488